PISA – Optical Microscopes

Optical microscopes are available in the Microscopy Lab for different techniques, such as brightfield, polarization contrast, fluorescence. The microscopes can also be equipped with cameras.

We also have the FlowCam 5000 by Yokogawa, which is a particle analyzer capable of taking optical images. It is comparable to a flow cytometer and features a syringe pump and 100 x 700 µm glass capillary, where liquid passes through. During a sample run, images of the flow are taken with a camera, magnified with a 10x objective. Detected particles are compiled as images in a collage, which can then be grouped and classified by the user based on the particle properties (e.g. size description and morphology, among others) provided by the software. Sample volume ranges from 0.25 to 1 mL.

Categories

Disciplinary Keywords

Instrumentation

Laboratory instrumentation

Instruments

  • Optical Microscope
  • Particle Analyzer

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